Endoscopic tissue characterization by frequency-domain fluorescence lifetime imaging (FD-FLIM)
نویسندگان
چکیده
منابع مشابه
Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens.
We report a side viewing fibre-based endoscope that is compatible with intravascular imaging and fluorescence lifetime imaging microscopy (FLIM). The instrument has been validated through testing with fluorescent dyes and collagen and elastin powders using the Laguerre expansion deconvolution technique to calculate the fluorescence lifetimes. The instrument has also been tested on freshly excis...
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Fluorescence Lifetime Imaging Microscopy (FLIM) allows fluorescence lifetime images of biological objects to be collected at 250 nm spatial resolution and at (sub-)nanosecond temporal resolution. Often ncomp kinetic processes underlie the observed fluorescence at all locations, but the intensity of the fluorescence associated with each process varies per-location, i.e., per-pixel imaged. Then t...
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During the last two decades, the use of fluorescence in natural sciences has propelled a number of research fields, ranging from biological sciences and applications in the clinical sector (FISH for genetic testing, advanced sequencing technology) to environmental monitoring. In addition, the resulting technological developments have become important in several fields of materials sciences, for...
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The fluorescence lifetime is a function of the refractive index of the fluorophore’s environment, for example in the case of the biologically important green fluorescent protein (GFP). In order to address the question whether this effect can be exploited to image the local environment of specific proteins in cell biology, we need to determine the distance over which the fluorophore’s lifetime i...
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ژورنال
عنوان ژورنال: Lasers in Medical Science
سال: 1997
ISSN: 0268-8921,1435-604X
DOI: 10.1007/bf02765101